The line between "optimizing" and just "sensible design" is sometimes fairly fine, but other times pretty obvious. Just for example, you don't need a profiler to be pretty sure that if you're sorting a few million items, it's worth using an O(N log N) algorithm rather than an O(N2) algorithm. IMO, that just falls under being reasonable sensible, not optimization though.
There are also some things you might as well do, simply because they might provide a benefit, and the cost is minimal to nonexistent. To use @unholysampler's example, writing
++i instead of
i++ may have some minuscule cost if you're accustomed to typing it as a post-increment, but (at most) it's temporary and trivial. I wouldn't spend any time rewriting working code for the sake of possibly saving a nanosecond, unless the profiler had shown that I really needed that time, and stood a reasonable chance of saving there. At the same time, when I'm just typing in new code, I'd work at habitually using the form that's likely to be better, because once you do so habitually it's free. It frequently won't gain anything, and even when it makes a difference it often won't be large enough to notice or care about -- but it's still free, so there's not reason not to do it.
Cases like those are fairly obvious, and in my mind would really fall under sensible design rather than what I'd think of as truly optimization. Most other cases of "optimization without representation" would be considerably harder to justify though. If you're going to spend any significant time or effort on the optimization, you should have something much more solid than a "gut feel" to justify it.
I should add that part of the reason I say that is that I think profiling code is extremely useful, even when your goal isn't optimization. A profiler gives a high-level overview of a piece of code that can be extremely useful even when you don't particularly care about optimization at all. Just for example, if you see 10x as many calls to allocate a resource as to free that type of resource, it's a pretty good clue that there's a real problem, even if the code currently seems to run just fine. When you get down to it, a lot of code has a lot of things that should match up (not necessarily 1:1, but somehow or other) and a profiler can show mismatches like that much more quickly than most other tools.